GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

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NSJ Bioreagents
Product Code: NSJ-V8219
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8219-20UG20 ug£264.00
Quantity:
NSJ-V8219-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CPTC-GCLM-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the GCLM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) A549, 2) A431 and 3) HepG2 cell lysate with GCLM antibody. Expected molecular weight ~31 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free GCLM antibody as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) A431 and 3) HepG2 cell lysate with GCLM antibody. Expected molecular weight ~31 kDa.
SDS-PAGE analysis of purified, BSA-free GCLM antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the GCLM antibody should be determined by the researcher.
Description:
GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.
Limitation:
This GCLM antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P48507