CD21 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7754
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7754-20UG20 ug£264.00
Quantity:
NSJ-V7754-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: CR2/3124R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R, green) and Reddot nuclear stain (red).
2 / 4
Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R); Red=isotype control, Blue= recombinant CD21 antibody.
3 / 4
IHC staining of FFPE human tonsil with recombinant CD21 antibody (clone CR2/3124R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone CR2/3124R) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R); Red=isotype control, Blue= recombinant CD21 antibody.
IHC staining of FFPE human tonsil with recombinant CD21 antibody (clone CR2/3124R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone CR2/3124R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A partial protein corresponding to amino acids 142-240 was used as the immunogen for the recombinant CD21 antibody.
Limitation:
This recombinant CD21 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P20023