HLA-DP + HLA-DQ + HLA-DR Antibody (MHC II)

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NSJ Bioreagents
Product Code: NSJ-V3374
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3374-20UG20 ug£264.00
Quantity:
NSJ-V3374-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CR3/43
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the HLA-DP/DQ/DR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
2 / 5
IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
3 / 5
IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).
4 / 5
Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10^6 cells (blocked with goat sera); Red=isotype control, Blue= HLA-DP/DQ/DR antibody.
5 / 5
SDS-PAGE analysis of purified, BSA-free HLA-DP/DQ/DR antibody (clone CR3/43) as confirmation of integrity and purity.
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IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10^6 cells (blocked with goat sera); Red=isotype control, Blue= HLA-DP/DQ/DR antibody.
SDS-PAGE analysis of purified, BSA-free HLA-DP/DQ/DR antibody (clone CR3/43) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
Description:
This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
Limitation:
This HLA-DP/DQ/DR antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04440, P01908, P01909, P01920, P01903