KRT10 Antibody / Cytokeratin 10

NSJ Bioreagents
Product Code: NSJ-V7113
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7113-20UG20 ug£264.00
Quantity:
NSJ-V7113-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTKN10-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Keratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human thymus lysate with Keratin 10 antibody (clone CTKN10-1). Predicted molecular weight ~59 kDa.~
2 / 2
IHC testing of FFPE human bladder carcinoma and Keratin 10 antibody. Staining of formalin-fixed tissues requires

Western blot testing of human thymus lysate with Keratin 10 antibody (clone CTKN10-1). Predicted molecular weight ~59 kDa.~
IHC testing of FFPE human bladder carcinoma and Keratin 10 antibody. Staining of formalin-fixed tissues requires

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the Keratin 10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Keratin-10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. Keratin 10 has been shown to interact with AKT1. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.
Limitation:
This Keratin 10 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P13645