KRT10 Antibody / Cytokeratin 10

NSJ Bioreagents
Product Code: NSJ-V3711
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3711-20UG20 ug£264.00
Quantity:
NSJ-V3711-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CTKN10-2R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Cytokeratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 1
IHC testing of FFPE human skin with recombinant Cytokeratin 10 antibody (clone CTKN10-2R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human skin with recombinant Cytokeratin 10 antibody (clone CTKN10-2R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Further Information

Application Details :
Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cytokeratin-10 (CK-10) or keratin-10 (K10) is a protein that in humans is encoded by the KRT10 gene. CK10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the recombinant Cytokeratin 10 antibody.
Limitation:
This recombinant Cytokeratin 10 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P13645