Cytokeratin 18 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7110
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7110-20UG20 ug£264.00
Quantity:
NSJ-V7110-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTKN18-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone CTKN18-1, green) and Reddot nuclear stain (red).
2 / 3
IHC testing of FFPE human lung carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.
3 / 3
Western blot testing of HCT-116 cell lysate with Cytokeratin 18 antibody (clone CTKN18-1). Predicted molecular weight ~48 kDa.
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Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone CTKN18-1, green) and Reddot nuclear stain (red).
IHC testing of FFPE human lung carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.
Western blot testing of HCT-116 cell lysate with Cytokeratin 18 antibody (clone CTKN18-1). Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the Cytokeratin 18 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.
Limitation:
This Cytokeratin 18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P05783