Multi Cytokeratin Antibody

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NSJ Bioreagents
Product Code: NSJ-V3718
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3718-20UG20 ug£264.00
Quantity:
NSJ-V3718-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: CTKNM-1R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone CTKNM-1R). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone CTKNM-1R). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human epithelial cell keratin was used as the immunogen for the recombinant Multi Cytokeratin antibody.
Limitation:
This recombinant Multi Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human