NSJ Bioreagents

Cytokeratin 18 Antibody

Product Code:
 
NSJ-V2671
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DE-K18
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Flow cytometry testing of MeOH fixed human HeLa cells with Cytokeratin 18 antibody (clone DE-K18); Red=isotype control, Blue= Cytokeratin 18 antibody.
2 / 4
IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (clone DE-K18).
3 / 4
Western blot testing of HCT116 cell lysate with Cytokerain 18 antibody (clone DE-K18). Expected molecular weight: 46-50 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone DE-K18) as confirmation of integrity and purity.

Flow cytometry testing of MeOH fixed human HeLa cells with Cytokeratin 18 antibody (clone DE-K18); Red=isotype control, Blue= Cytokeratin 18 antibody.
IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (clone DE-K18).
Western blot testing of HCT116 cell lysate with Cytokerain 18 antibody (clone DE-K18). Expected molecular weight: 46-50 kDa.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone DE-K18) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V2671-20UG20 ug£264.00
Quantity:
NSJ-V2671-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Cytokeratin 18 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A cytoskeletal preparation from A431 cells was used as the immunogen for the Cytokeratin 18 antibody.
Limitation:
This Cytokeratin 18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P05783