NSJ Bioreagents

Basic Cytokeratin Antibody (HMW / Type II)

Product Code:
 
NSJ-V8314
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DE-SQ
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of FFPE human tonsil with Basic Cytokeratin antibody (clone DE-SQ). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with Basic Cytokeratin antibody (clone DE-SQ). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V8314-20UG20 ug£264.00
Quantity:
NSJ-V8314-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction was used as the immunogen for this Basic Cytokeratin antibody.
Limitation:
This Basic Cytokeratin antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q01546