Melanoma Marker Antibody Cocktail (MART-1 & gp100)

NSJ Bioreagents
Product Code: NSJ-V8320
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8320-20UG20 ug£264.00
Quantity:
NSJ-V8320-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa + Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: DT101+ BC199 + HMB45
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Melanoma Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
IHC staining of FFP

IHC staining of FFPE human melanoma tissue with Melanoma Marker antibody cocktail (clones DT101+ BC199 + HMB45). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
IHC staining of FFP

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant hMART-1 protein (DT101 & BC199) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogen for this Melanoma Marker antibody cocktail.
Limitation:
This Melanoma Marker antibody cocktail is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Human