Cyclin A2 Antibody / CCNA2

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.

This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk�s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk�s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

890

Full length bovine protein was used as the immunogen for this CCNA2 antibody. The epitope has been localized to amino acids 144-148 of the human protein.

This Cyclin A2 antibody is available for research use only.

Predominantly nuclear

Protein G affinity chromatography

Human, Mouse