EpCAM Antibody / Extracellular domain

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NSJ Bioreagents
Product Code: NSJ-V3358
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3358-20UG20 ug£264.00
Quantity:
NSJ-V3358-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG
Antibody Clonality: Monoclonal
Antibody Clone: EGP40/1384
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Feline (cat)
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
IHC testing of FFPE human ovarian carcinoma and EpCAM antibody (clone EGP40/1384). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
2 / 5
Immunofluorescent staining of human MCF-7 cells with EpCAM antibody (green, clone EGP40/1384) and Reddot nuclear stain (red).
3 / 5
IHC testing of FFPE dog colon and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.
4 / 5
IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.
5 / 5
IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.
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IHC testing of FFPE human ovarian carcinoma and EpCAM antibody (clone EGP40/1384). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
Immunofluorescent staining of human MCF-7 cells with EpCAM antibody (green, clone EGP40/1384) and Reddot nuclear stain (red).
IHC testing of FFPE dog colon and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.

Documents

Further Information

Application Details :
ELISA: order BSA/sodium azide-free format for coating,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the EpCAM antibody may be required for optimal performance.
Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A human partial recombinant protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the EpCAM antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Dog, Cat
Uniprot #:
P16422