EpCAM Antibody

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NSJ Bioreagents
Product Code: NSJ-V3463
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3463-20UG20 ug£264.00
Quantity:
NSJ-V3463-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: EGP40/1798
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Feline (cat)
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Ep-CAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of human MCF-7 cells with Ep-CAM antibody (green, clone EGP40/1798) and Reddot nuclear stain (red).
2 / 5
Western blot testing of human MCF7 cell lysate with Ep-CAM antibody (clone EGP40/1798). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
3 / 5
IHC testing of FFPE human colorectal carcinoma and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
4 / 5
IHC testing of FFPE dog bladder and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
5 / 5
IHC testing of FFPE cat small intestine and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
❮❯

Immunofluorescent staining of human MCF-7 cells with Ep-CAM antibody (green, clone EGP40/1798) and Reddot nuclear stain (red).
Western blot testing of human MCF7 cell lysate with Ep-CAM antibody (clone EGP40/1798). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
IHC testing of FFPE human colorectal carcinoma and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
IHC testing of FFPE dog bladder and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
IHC testing of FFPE cat small intestine and Ep-CAM antibody (clone EGP40/1798). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Documents

Further Information

Application Details :
ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Titering of the Ep-CAM antibody may be required for optimal performance.
Description:
EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A human recombinant partial protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
Limitation:
This Ep-CAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Dog, Cat
Uniprot #:
P16422