EpCAM Antibody
Code | Size | Price |
---|
NSJ-V3117S-0.1ML | 0.1 ml | £264.00 |
Quantity:
NSJ-V3117S-0.5ML | 0.5 ml | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: Epcam/1158
Regulatory Status: RUO
Target Species:
- Mouse
- Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the rat EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details :
Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (3)
Application Note:
Optimal dilution of the rat EpCAM antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Format :
Bioreactor concentrate
Formulation :
Bioreactor concentrate with 0.05% sodium azide
Immunogen:
Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.
Limitation:
This rat EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Unpurified high titer supernatant
Species Reactivity :
Rat and Mouse. Does not react with Human. Others species not known.
Uniprot #:
O55159