EpCAM Antibody

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NSJ Bioreagents
Product Code: NSJ-V7010
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7010-20UG20 ug£264.00
Quantity:
NSJ-V7010-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: EPM17-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EPM17-1, green) and Reddot nuclear stain (red).
2 / 3
IHC testing of FFPE human colon carcinoma and EpCAM antibody (EPM17-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
3 / 3
Western blot testing of HCT116 cell lysate and EpCAM antibody (EPM17-1). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
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Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EPM17-1, green) and Reddot nuclear stain (red).
IHC testing of FFPE human colon carcinoma and EpCAM antibody (EPM17-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Western blot testing of HCT116 cell lysate and EpCAM antibody (EPM17-1). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 2-4ug/ml,Immunofluorescence: 1-2 ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
Application Note:
Titering of the EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the EpCAM antibody.
Limitation:
This EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16422