Estrogen Receptor alpha Antibody
Code | Size | Price |
---|
NSJ-V2113-20UG | 20 ug | £264.00 |
Quantity:
NSJ-V2113-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ER506
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the ER antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details :
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)
Application Note:
Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.
1. FFPE testing requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and cooling at RT for 20 minutes.
Description:
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
2099
Immunogen:
Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
Limitation:
This ER antibody is available for research use only.
Localization:
Intracellular, Nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human