Estrogen Receptor alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-V3843
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3843-20UG20 ug£264.00
Quantity:
NSJ-V3843-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ESR1/1935
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of human endometrial carcinoma stained with Estrogen Receptor alpha antibody (clone ESR1/1935). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2 / 3
IHC testing of human breast carcinoma stained with Estrogen Receptor alpha antibody (clone ESR1/1935). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
3 / 3
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/1935) as confirmation of integrity and purity.
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IHC testing of human endometrial carcinoma stained with Estrogen Receptor alpha antibody (clone ESR1/1935). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
IHC testing of human breast carcinoma stained with Estrogen Receptor alpha antibody (clone ESR1/1935). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/1935) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the Estrogen Receptor alpha antibody for each application should be determined by the researcher.
Description:
This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Full length human protein was used as the immunogen for this Estrogen Receptor alpha antibody.
Limitation:
This Estrogen Receptor alpha antibody is available for research use only.
Localization:
Nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P03372