Estrogen Receptor alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-V3581
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3581-20UG20 ug£264.00
Quantity:
NSJ-V3581-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: ESR1/2299R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Estrogen Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human breast cancer with Estrogen Receptor antibody (clone ESR1/2299R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min. ~
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Western blot testing of h
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IHC testing of FFPE human breast cancer with Estrogen Receptor antibody (clone ESR1/2299R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min. ~
Western blot testing of h

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The optimal dilution of the recombinant Estrogen Receptor antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor antibody.
Limitation:
This recombinant Estrogen Receptor antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P03372