Filaggrin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V3840BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3840BTN-500ul500 ul£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: FLG/1563
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Filaggrin antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Images

1 / 3
IHC testing of FFPE human skin with biotinylated Filaggrin antibody (clone FLG/1563). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
2 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Filaggrin antibody (clone FLG/1563). These results demonstrate the foremost specificity of the FLG/1563 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
3 / 3
SDS-PAGE analysis of purified, BSA-free Filaggrin antibody (clone FLG/1563) as confirmation of integrity and purity.
❮❯

IHC testing of FFPE human skin with biotinylated Filaggrin antibody (clone FLG/1563). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Filaggrin antibody (clone FLG/1563). These results demonstrate the foremost specificity of the FLG/1563 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Filaggrin antibody (clone FLG/1563) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Filaggrin antibody should be determined by the researcher.
Description:
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Format :
Biotin Conjugate
Formulation :
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 998-1104 from the human protein was used as the immunogen for the Filaggrin antibody.
Limitation:
This Filaggrin antibody is available for research use only.
Localization:
Cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P20930