Filaggrin Antibody

NSJ Bioreagents
Product Code: NSJ-V3861
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3861-20UG20 ug£264.00
Quantity:
NSJ-V3861-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: FLG1-1
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 1
IHC testing of FFPE human skin with Filaggrin antibody (clone FLG1-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human skin with Filaggrin antibody (clone FLG1-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The stated application concentrations are suggested starting points. Titration of the Filaggrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Filaggrin (FLG) aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 998-1104 from the human protein were used as the immunogen for this Filaggrin antibody.
Limitation:
This Filaggrin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P20930