FOXA1 Antibody
| Code | Size | Price |
|---|
| NSJ-V3620-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V3620-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: FOXA1/2230R
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store the recombinant FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Documents
Further Information
Application Details :
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-2ug/million cells
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant FOXA1 antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
The transcription factor Forkhead-box A1, also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (a and beta), HNF-3 (a, beta and gamma), HNF-4 (a and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. Its expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this recombinant FOXA1 antibody.
Limitation:
This recombinant FOXA1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P55317