FTL Antibody / Ferritin light chain

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NSJ Bioreagents
Product Code: NSJ-V3351
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3351-20UG20 ug£264.00
Quantity:
NSJ-V3351-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: FTL/1386
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Ferritin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 6
IHC staining of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 6
Western blot testing of human HeLa lysate with Ferritin Light Chain antibody (clone FTL/1386). Predicted molecular weight: ~20 kDa.
3 / 6
IHC staining of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human testicular carcinoma with Ferritin Light Chain antibody (clone FTL/1386). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) A431, 2) HeLa, 3) liver and 4) testis lysate with Ferritin Light Chain antibody (clone FTL/1386). Predicted molecular weight: ~20 kDa.
6 / 6
Western blot testing of human kidney lysate with recombinant FTL antibody (clone FTL/1386). Predicted molecular weight: ~20 kDa.
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IHC staining of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human HeLa lysate with Ferritin Light Chain antibody (clone FTL/1386). Predicted molecular weight: ~20 kDa.
IHC staining of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human testicular carcinoma with Ferritin Light Chain antibody (clone FTL/1386). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human 1) A431, 2) HeLa, 3) liver and 4) testis lysate with Ferritin Light Chain antibody (clone FTL/1386). Predicted molecular weight: ~20 kDa.
Western blot testing of human kidney lysate with recombinant FTL antibody (clone FTL/1386). Predicted molecular weight: ~20 kDa.

Documents

Further Information

Application Details :
ELISA: order BSA/sodium azide-free format for coating,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin Light Chain antibody to be titered up or down for optimal performance.
Description:
Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.
Limitation:
This Ferritin Light Chain antibody is available for research use only.
Localization:
Cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P02792