FTL Antibody / Ferritin light chain

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

Optimal dilution of the FTL antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Amino acids 38-165 from the human protein were used as the immunogen for this FTL antibody.

This FTL antibody is available for research use only.

Cytoplasmic

Yes

Protein G affinity chromatography

Human

P02792