CD15 / SSEA-1 Antibody Cocktail

Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Optimal dilution of the SSEA-1 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

SSEA-1/CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, it is expressed in Reed-Sternberg cells and some epithelial cells. SSEA-1/CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Purified human neutrophils (FUT4/815) and Myelomonocytic leukemia cells (BRA-4F1) were used as the immunogen for the SSEA-1 antibody cocktail.

This SSEA-1 antibody is available for research use only.

Cytoplasmic and cell surface

PEG precipitation

Human, Mouse, Rat

P22083