Galectin 1 Antibody / LGALS1

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NSJ Bioreagents
Product Code: NSJ-V3201
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3201-20UG20 ug£264.00
Quantity:
NSJ-V3201-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, lambda
Antibody Clonality: Monoclonal
Antibody Clone: GAL1/1831
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Galectin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Western blot testing of human 1) JEG-3 and 2) K562 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
2 / 5
Western blot testing of human HeLa cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
3 / 5
IHC testing of FFPE human prostate carcinoma with Galectin 1 antibody (clone GAL1/1831). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Galectin 1 antibody (clone GAL1/1831). These results demonstrate the foremost specificity of the GAL1/1831 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free Galectin 1 antibody (clone GAL1/1831) as confirmation of integrity and purity.
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Western blot testing of human 1) JEG-3 and 2) K562 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
Western blot testing of human HeLa cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.
IHC testing of FFPE human prostate carcinoma with Galectin 1 antibody (clone GAL1/1831). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Galectin 1 antibody (clone GAL1/1831). These results demonstrate the foremost specificity of the GAL1/1831 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Galectin 1 antibody (clone GAL1/1831) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA: 2-4ug/ml (order BSA/azide-free format),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Titering of the Galectin 1 antibody may be required for optimal performance.
Description:
Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody.
Limitation:
This Galectin 1 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P09382