GAPDH Antibody Loading Control
| Code | Size | Price |
|---|
| NSJ-N1074-25UG | 25 ug | £254.00 |
Quantity:
| NSJ-N1074-100UG | 100 ug | £450.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: GAP259
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Non-Human Primates
- Rat
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot and store the loading control antibody frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Western blot: 0.5-1ug/ml,IHC (FFPE): 3-5ug/ml
Application Note:
Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.
Buffer:
1X PBS, pH 7.4
Description:
Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Format :
Purified
Formulation :
0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
2597
Immunogen:
A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.
Limitation:
This GAPDH antibody loading control is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G Affinity Chromatography
Species Reactivity :
Human, Mouse, Rat, Primate, Dog