EGF Receptor / EGFR Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V3828
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3828-20UG20 ug£264.00
Quantity:
NSJ-V3828-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: GFR/2341
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
Western blot testing of A431 lysate using EGFR antibody (clone GFR/2341). Expected molecular weight: 134-180 kDa depending on glycosylation level.
2 / 3
IHC testing of FFPE human placenta with EGFR antibody (clone GFR/2341). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EGFR antibody (clone GFR/2341). These results demonstrate the foremost specificity of the GFR/2341 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Western blot testing of A431 lysate using EGFR antibody (clone GFR/2341). Expected molecular weight: 134-180 kDa depending on glycosylation level.
IHC testing of FFPE human placenta with EGFR antibody (clone GFR/2341). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EGFR antibody (clone GFR/2341). These results demonstrate the foremost specificity of the GFR/2341 mAb.<br>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Titering of the EGFR antibody may be required for optimal performance.
Description:
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 344-492 from the human protein was used as the immunogen for the EGFR antibody.
Limitation:
This EGFR antibody is available for research use only.
Localization:
Cell surface
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P00533