Glypican 3 Antibody / GPC3

NSJ Bioreagents
Product Code: NSJ-V7261
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7261-20UG20 ug£264.00
Quantity:
NSJ-V7261-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: GPC3/1534R
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Glypican-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of MeOH fixed human HepG2 cells with recombinant Glypican-3 antibody (clone GPC3/1534R, green) and Reddot nuclear stain (red).~
2 / 2
IHC testing of FFPE human fetal liver tissue with recombinant Glypican-3 antibody

Immunofluorescent staining of MeOH fixed human HepG2 cells with recombinant Glypican-3 antibody (clone GPC3/1534R, green) and Reddot nuclear stain (red).~
IHC testing of FFPE human fetal liver tissue with recombinant Glypican-3 antibody

Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A full length human protein was used as the immunogen for the recombinant Glypican-3 antibody.
Limitation:
This recombinant Glypican-3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P51654