CD44 Antibody

NSJ Bioreagents
Product Code: NSJ-F40263
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40263-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40263-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a , k
Antibody Clonality: Monoclonal
Antibody Clone: Hermes-3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the CD44 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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CD44 antibody western blot analysis in HeLa lysate
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CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma.
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CD44 antibody confocal immunofluorescent analysis with HeLa cells. Primary Ab was followed by PE secondary (red). DAPI was used to stain the cell nucleus (blue).
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CD44 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram).

CD44 antibody western blot analysis in HeLa lysate
CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma.
CD44 antibody confocal immunofluorescent analysis with HeLa cells. Primary Ab was followed by PE secondary (red). DAPI was used to stain the cell nucleus (blue).
CD44 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram).

Further Information

Application Details :
Western blot: 1:100-1:500,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CD44 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This CD44 antibody was produced from a mouse immunized with recombinant protein.
Limitation:
This CD44 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P16070