HLA-DPB1 Antibody (MHC II)

NSJ Bioreagents
Product Code: NSJ-V7419
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7419-20UG20 ug£264.00
Quantity:
NSJ-V7419-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: HLA-DPB1/2862R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Store the recombinant HLA-DPB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescent staining of human Raji cells with recombinant HLA-DPB1 antibody (clone HLA-DPB1/2862R, green) and Reddot nuclear stain (red).~
2 / 2
Flow cytometry testing of human Raji cells with recombinant HLA-DPB1 antibody; Red=isotype c

Immunofluorescent staining of human Raji cells with recombinant HLA-DPB1 antibody (clone HLA-DPB1/2862R, green) and Reddot nuclear stain (red).~
Flow cytometry testing of human Raji cells with recombinant HLA-DPB1 antibody; Red=isotype c

Further Information

Application Details :
ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the recombinant HLA-DPB1 antibody should be determined by the researcher.
Description:
Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the recombinant HLA-DPB1 antibody.
Limitation:
This recombinant HLA-DPB1 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04440