HNF1A Antibody

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NSJ Bioreagents
Product Code: NSJ-V7353
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7353-20UG20 ug£264.00
Quantity:
NSJ-V7353-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: HNF1A/2087
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the HNF1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human pancreas with HNF1A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human pancreas with HNF1A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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SDS-PAGE analysis of purified, BSA-free HNF1A antibody as confirmation of integrity and purity.
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IHC staining of FFPE human pancreas with HNF1A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human pancreas with HNF1A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free HNF1A antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA: order BSA/sodium azide-free format for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the HNF1A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
HNF1A belongs to the homeobox protein family and is an essential transcription factor for many hepatic genes involved in detoxification, homeostasis and metabolisms of glucose, lipid, steroid and amino acid. In addition, HNF1A is an important component of the transcriptional networks governing embryonic pancreas development and differentiation. as well as maintaining the growth and function of islet cells in adult. HNF1A (Hepatocyte nuclear factor 1 alpha) is a transcription factor that is known to regulate pancreatic differentiation and maintain homeostasis of endocrine pancreas. Recently, genome-wide association studies have implicated HNF1A as a susceptibility gene for pancreatic cancer.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 214-339 from the human protein was used as the immunogen for the HNF1A antibody.
Limitation:
This HNF1A antibody is available for research use only.
Localization:
Nuclear
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P20823