CD57 / HNK-1 Antibody Cocktail

Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the CD57 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human peripheral blood mononuclear cells were used as the immunogen for the CD57 antibody cocktail.

This CD57 antibody cocktail is available for research use only.

Cell surface, cytoplasmic

PEG precipitation

Human

Q9P2W7