ICOS-L Antibody / ICOS Ligand / B7RP-1

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NSJ Bioreagents
Product Code: NSJ-V7774
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7774-20UG20 ug£264.00
Quantity:
NSJ-V7774-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ICOSL/3260
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the ICOS Ligand antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC staining of FFPE human colon carcinoma with ICOS Ligand antibody (clone ICOSL/3260). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ICOS Ligand antibody (clone ICOSL/3260). These results demonstrate the foremost specificity of the ICOSL/3260 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
3 / 3
SDS-PAGE analysis of purified, BSA-free ICOS Ligand antibody (clone ICOSL/3260) as confirmation of integrity and purity.
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IHC staining of FFPE human colon carcinoma with ICOS Ligand antibody (clone ICOSL/3260). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ICOS Ligand antibody (clone ICOSL/3260). These results demonstrate the foremost specificity of the ICOSL/3260 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free ICOS Ligand antibody (clone ICOSL/3260) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This MAb recognizes a protein of 36kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 23-149) was used as the immunogen for the ICOS Ligand antibody.
Limitation:
This ICOS Ligand antibody is available for research use only.
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
O75144