IgG Heavy Chain Antibody Cocktail

Flow cytometry: 1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

The optimal dilution of the IgG antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Purified human IgG was used as the immunogen for this IgG antibody.

This IgG antibody cocktail is available for research use only.

Cytoplasm, Cell Surface and Secreted

Protein G affinity chromatography

Human

P01857, P01859, P01860, P01861