Integrin beta 3 Antibody / ITGB3 / CD61

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Titration of the recombinant Integrin beta 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Reacts with human Integrin beta 3, also called CD61. It associates with the alphaV-chain (CD51) to form vitronectin receptor, or with the alpha-IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human recombinant protein was used as the immunogen for the recombinant Integrin beta 3 antibody.

This recombinant Integrin beta 3 antibody is available for research use only.

Cell surface, cytoplasmic

Protein A affinity

Human

P05106