CD79a Antibody

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NSJ Bioreagents
Product Code: NSJ-V2078
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2078-20UG20 ug£264.00
Quantity:
NSJ-V2078-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: JCB117
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
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IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
3 / 7
IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
4 / 7
Immunofluorescent staining of PFA-fixed human Raji cells with CD79 antibody (clone JCB117, green) and Reddot nuclear stain (red).
5 / 7
Flow cytometry testing of human Raji cells with CD79a antibody (clone JCB117); Red=isotype control, Blue= CD79a antibody.
6 / 7
SDS-PAGE analysis of purified, BSA-free CD79a antibody (clone JCB117) as confirmation of integrity and purity.
7 / 7
Western blot testing of two different lots of human Raji cell lysate with CD79a antibody (clone JCB117). Expected molecular weight: 25-47 kDa depending on glycosylation level.
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IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
IHC testing of FFPE human tonsil stained with CD79a antibody (clone JCB117). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Immunofluorescent staining of PFA-fixed human Raji cells with CD79 antibody (clone JCB117, green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with CD79a antibody (clone JCB117); Red=isotype control, Blue= CD79a antibody.
SDS-PAGE analysis of purified, BSA-free CD79a antibody (clone JCB117) as confirmation of integrity and purity.
Western blot testing of two different lots of human Raji cell lysate with CD79a antibody (clone JCB117). Expected molecular weight: 25-47 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD79a antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B-cell Ag receptor. CD79a first appears at pre B-cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. It is found in the majority of acute leukemias of B-cell precursors, lines and lymphomas, and in some myelomas. It is not present in myeloid or Tcell lines. CD79a antibody is generally used to complement CD20 mAb, especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This CD79a antibody will stain many of the same lymphomas as CD20 mAb, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 mAb. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
973
Immunogen:
Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for this CD79a antibody.
Limitation:
This CD79a antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human