Cytokeratin 8/18 Antibody Cocktail

Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Differences in protocols and secondaries may require the Cytokeratin 8/18 antibody to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Cytokeratins are a group of proteins expressed in pairs in keratinized and non-keratinized epithelial tissue. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). The pair are normally seen in simple but not stratified epithelial cells. This antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this antibody cocktail is indistinguishable from that obtained with monoclonal antibody 5D3.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

3856

A keratin preparation from a human carcinoma (K8.8) and PMC-42 human breast carcinoma cells (DC10) were used as the immunogen for this Cytokeratin 8/18 antibody cocktail.

This Cytokeratin 8/18 antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

Human