Cytokeratin 14 Antibody / CK14 / KRT14

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NSJ Bioreagents
Product Code: NSJ-V7917
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7917-20UG20 ug£264.00
Quantity:
NSJ-V7917-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: KRT14/2375
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Cytokeratin 14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 14 antibody (clone KRT14/2375); Red=isotype control, Blue= Cytokeratin 14 antibody.
2 / 5
IHC staining of FFPE human skin with Cytokeratin 14 antibody (clone KRT14/2375). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human prostate carcinoma with Cytokeratin 14 antibody (clone KRT14/2375). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human prostate carcinoma with Cytokeratin 14 antibody (clone KRT14/2375). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free Cytokeratin 14 antibody (clone KRT14/2375) as confirmation of integrity and purity.
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Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 14 antibody (clone KRT14/2375); Red=isotype control, Blue= Cytokeratin 14 antibody.
IHC staining of FFPE human skin with Cytokeratin 14 antibody (clone KRT14/2375). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with Cytokeratin 14 antibody (clone KRT14/2375). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with Cytokeratin 14 antibody (clone KRT14/2375). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 14 antibody (clone KRT14/2375) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 351-472) was used as the immunogen for this Cytokeratin 14 antibody.
Limitation:
This Cytokeratin 14 antibody is available for research use only.
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat