Keratin 6 Antibody / Cytokeratin 6

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NSJ Bioreagents
Product Code: NSJ-V3850
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3850-20UG20 ug£264.00
Quantity:
NSJ-V3850-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: KRT6A/2368
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the Cytokeratin 6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human basal cell carcinoma with Cytokeratin 6 antibody (clone KRT6A/2368). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human basal cell carcinoma with Cytokeratin 6 antibody (clone KRT6A/2368). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 4
SDS-PAGE analysis of purified, BSA-free Cytokeratin 6A antibody (clone KRT6A/2368) as confirmation of integrity and purity.
4 / 4
Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 6 antibody (clone KRT6A/2368); Red=isotype control, Blue= Cytokeratin 6 antibody.
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IHC testing of FFPE human basal cell carcinoma with Cytokeratin 6 antibody (clone KRT6A/2368). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human basal cell carcinoma with Cytokeratin 6 antibody (clone KRT6A/2368). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 6A antibody (clone KRT6A/2368) as confirmation of integrity and purity.
Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 6 antibody (clone KRT6A/2368); Red=isotype control, Blue= Cytokeratin 6 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Cytokeratin 6 antibody should be determined by the researcher.
Description:
This mAb recognizes a protein of 56kDa, identified as Cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human KRT6A protein was used as the immunogen for the Cytokeratin 6 antibody.
Limitation:
This Cytokeratin 6 antibody is available for research use only.
Localization:
Cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P02538