HSP60 Antibody

NSJ Bioreagents
Product Code: NSJ-V2137
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V2137-20UG20 ug£264.00
Quantity:
NSJ-V2137-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: LK2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 3
IHC staining of FFPE human liver tissue with HSP60 antibody (clone LK2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone LK2, green) and Reddot nuclear stain (red).
3 / 3
Western blot testing of Hep-2 cell lysate with HSP60 antibody (clone LK2).

IHC staining of FFPE human liver tissue with HSP60 antibody (clone LK2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone LK2, green) and Reddot nuclear stain (red).
Western blot testing of Hep-2 cell lysate with HSP60 antibody (clone LK2).

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). The antibody's epitope is localized between amino acids 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK2 antibody, unlike LK1 antibody, recognizes both mammalian and bacterial HSP60.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
3329
Immunogen:
Recombinant human HSP60 protein was used as the immunogen for this antibody.
Limitation:
This HSP60 antibody is available for research use only.
Localization:
Mitochondria in cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human