RPSA Antibody / 40S Ribosomal protein SA

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NSJ Bioreagents
Product Code: NSJ-V7289
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7289-20UG20 ug£264.00
Quantity:
NSJ-V7289-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG
Antibody Clonality: Monoclonal
Antibody Clone: LMNR-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the RPSA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human HeLa cell lysate with RPSA antibody (clone LMNR-1). Routinely observed molecular weight: 37-40 kDa and 67 kDa.
2 / 3
IHC testing of FFPE human tonsil stained with RPSA antibody (clone LMNR-1). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 3
FACS staining of PFA-fixed Raji cells with RPSA antibody (clone LMNR-1); Red=isotype control, Blue= RPSA antibody.
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Western blot testing of human HeLa cell lysate with RPSA antibody (clone LMNR-1). Routinely observed molecular weight: 37-40 kDa and 67 kDa.
IHC testing of FFPE human tonsil stained with RPSA antibody (clone LMNR-1). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
FACS staining of PFA-fixed Raji cells with RPSA antibody (clone LMNR-1); Red=isotype control, Blue= RPSA antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the RPSA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full length protein was used as the immunogen for the RPSA antibody.
Limitation:
This RPSA antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08865