Connexin 32 Antibody / GJB1

Immunofluorescence: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Western blot: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane a-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

This Connexin 32 antibody is available for research use only.

Cell surface, cytoplasmic

Protein G affinity chromatography

Human, Mouse, Rat

P08034