MBP Antibody / Myelin Basic Protein

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NSJ Bioreagents
Product Code: NSJ-V8676
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8676-20UG20 ug£264.00
Quantity:
NSJ-V8676-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: MBP/4272
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the MBP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human brain lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.
4 / 5
Western blot testing of human U-87 MG cell lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.
5 / 5
SDS-PAGE analysis of purified, BSA-free MBP antibody as confirmation of integrity and purity.
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IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.
Western blot testing of human U-87 MG cell lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.
SDS-PAGE analysis of purified, BSA-free MBP antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the MBP antibody should be determined by the researcher.
Description:
Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin. MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids GAEGQRPGFGYGG were used as the immunogen for the MBP antibody.
Limitation:
This MBP antibody is available for research use only.
Localization:
Cell surface, cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P02686