MBP Antibody / Myelin Basic Protein

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NSJ Bioreagents
Product Code: NSJ-V8677
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8677-20UG20 ug£264.00
Quantity:
NSJ-V8677-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: Rat IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: MBP/4273
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Myelin Basic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human brain lysate with Myelin Basic Protein antibody (clone MBP/4273). Isoforms may be visualized from 20~37 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free Myelin Basic Protein antibody (clone MBP/4273) as confirmation of integrity and purity.
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IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human brain lysate with Myelin Basic Protein antibody (clone MBP/4273). Isoforms may be visualized from 20~37 kDa.
SDS-PAGE analysis of purified, BSA-free Myelin Basic Protein antibody (clone MBP/4273) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
Description:
Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4273 recognizes an epitope in the 82-87 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP. MBP/4273 is also used in a 2-site ELISA with clone MBP/4275. Both are used interchangeably as capture and detection antibody.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
The epitope for this Myelin Basic Protein antibody has been mapped to the 82-87 amino acid region (DENPVV).
Limitation:
This Myelin Basic Protein antibody is available for research use only.
Localization:
Cell surface, cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P02686