Beta-2 Microglobulin Antibody

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NSJ Bioreagents
Product Code: NSJ-V7123
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7123-20UG20 ug£264.00
Quantity:
NSJ-V7123-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MGBP2-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the B2M antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human renal carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2 / 4
IHC testing of FFPE human bladder carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
3 / 4
Flow cytometry testing of human HeLa cells with B2M antibody (clone MGBP2-1); Red=isotype control, Blue= B2M antibody.
4 / 4
Western blot testing of human Raji cell lysate with B2M antibody (clone MGBP2-1).
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IHC testing of FFPE human renal carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
IHC testing of FFPE human bladder carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Flow cytometry testing of human HeLa cells with B2M antibody (clone MGBP2-1); Red=isotype control, Blue= B2M antibody.
Western blot testing of human Raji cell lysate with B2M antibody (clone MGBP2-1).

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the B2M antibody may be required for optimal performance.

>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Beta-2 Microglobulin/B2M is a component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Full length recombinant human protein was used as the immunogen for the B2M antibody.
Limitation:
This B2M antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P61769