MITF Antibody
Code | Size | Price |
---|
NSJ-V7509-20UG | 20 ug | £264.00 |
Quantity:
NSJ-V7509-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: MITF/2987R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant MITF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Further Information
Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant MITF antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This mAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant MITF antibody.
Limitation:
This recombinant MITF antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
O75030