Mesothelin Antibody / MSLN

NSJ Bioreagents
Product Code: NSJ-V3821
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3821-20UG20 ug£264.00
Quantity:
NSJ-V3821-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MKPF-1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the MSLN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human lung mesothelioma with MSLN antibody (clone MKPF-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
IHC testing of FFPE rat stomach with MSLN anti

IHC testing of FFPE human lung mesothelioma with MSLN antibody (clone MKPF-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.~
IHC testing of FFPE rat stomach with MSLN anti

Further Information

Application Details :
IHC (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the MSLN antibody should be determined by the researcher.
Description:
Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 273-407 from the human protein was used as the immunogen for this MSLN antibody.
Limitation:
This MSLN antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13421