CD20 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7618
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7618-20UG20 ug£264.00
Quantity:
NSJ-V7618-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MS4A1/3411
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Images

1 / 4
Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3411) and Reddot nuclear stain (red).
2 / 4
IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3411). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 4
Western blot testing of human Raji lysate with CD20 antibody (clone MS4A1/3411). Predicted molecular weight ~33 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/3411) as confirmation of integrity and purity.
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Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3411) and Reddot nuclear stain (red).
IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3411). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human Raji lysate with CD20 antibody (clone MS4A1/3411). Predicted molecular weight ~33 kDa.
SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/3411) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Amino acids 213-297 from the human protein were used as the immunogen for this CD20 antibody.
Limitation:
This CD20 antibody is available for research use only.
Localization:
Predominantly cell surface with some cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P11836