MSH2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V7964
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7964-20UG20 ug£264.00
Quantity:
NSJ-V7964-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MSH2/2622
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the MSH2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human colon carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human basal cell carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human thyroid carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human colon from a Lynch disease patient with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Western blot testing of human cell lysates with MSH2 antibody. Expected molecular weight: ~105 kDa.
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Flow cytometry testing of permeabilized human A549 cells with MSH2 antibody; Red=isotype control, Blue= MSH2 antibody.
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Immunofluorescent staining of permeabilized human MOLT-4 cells with MSH2 antibody (green) and Phalloidin (red).
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SDS-PAGE analysis of purified, BSA-free MSH2 antibody as confirmation of integrity and purity.
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IHC staining of FFPE human colon carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human basal cell carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon from a Lynch disease patient with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human cell lysates with MSH2 antibody. Expected molecular weight: ~105 kDa.
Flow cytometry testing of permeabilized human A549 cells with MSH2 antibody; Red=isotype control, Blue= MSH2 antibody.
Immunofluorescent staining of permeabilized human MOLT-4 cells with MSH2 antibody (green) and Phalloidin (red).
SDS-PAGE analysis of purified, BSA-free MSH2 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 327-427) was used as the immunogen for this MSH2 antibody.
Limitation:
This MSH2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P43246