Moesin Antibody

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NSJ Bioreagents
Product Code: NSJ-V3342
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3342-20UG20 ug£264.00
Quantity:
NSJ-V3342-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MSN/491
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Moesin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human melanoma with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
2 / 7
IHC testing of FFPE human placenta with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
3 / 7
Western blot testing of human HT29 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
4 / 7
SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/491). Confirmation of Integrity and Purity of the Antibody.
5 / 7
Western blot testing of human PC3 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
6 / 7
Flow cytometry testing of permeabilized human K562 cells with Moesin antibody (clone MSN/491); Red=isotype control, Blue= Moesin antibody.
7 / 7
Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone MSN/491, green) and Reddot nuclear stain (red).
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IHC testing of FFPE human melanoma with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human placenta with Moesin antibody (clone MSN/491). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Western blot testing of human HT29 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Moesin Antibody (clone MSN/491). Confirmation of Integrity and Purity of the Antibody.
Western blot testing of human PC3 cell lysate with Moesin antibody (clone MSN/491). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
Flow cytometry testing of permeabilized human K562 cells with Moesin antibody (clone MSN/491); Red=isotype control, Blue= Moesin antibody.
Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone MSN/491, green) and Reddot nuclear stain (red).

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the Moesin antibody may be required for optimal performance.
Description:
Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the Moesin antibody.
Limitation:
This Moesin antibody is available for research use only.
Localization:
Cytoplasmic & cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P26038